Objective of this research was to study protective effect of methanolic extract of Saraca indica bark (MESI) on doxorubicin induced hepatotoxicity and nephrotoxicity in Wistar rats. The animals were divided in following experimental groups, 5 animals in each. Group I: Vehicle (Distilled water 1 ml/kg, p.o.) for 21 days, Group II: Doxorubicin (DXR) (60 mg/kg, i.p.) once, 48 h before sacrifice, Group III: MESI (200 mg/kg, p.o.) for 21 days, Group IV: MESI (200 mg/kg, p.o.) for 21 days + DXR (60 mg/kg, i.p.) once, 48 h before sacrifice, Group V: MESI (400 mg/kg, p.o.) for 21 days + DXR (60 mg/kg, i.p.) once, 48 h before sacrifice, Group VI: Limarin (25 mg/kg, p.o.) for 21 days + DXR (60 mg/kg, i.p.) Once, 48 h before sacrifice. At the end of third week, DXR (60 mg/kg, i.p.) was given to the animals. After 21 days, animals were sacrificed and blood samples were collected for determination of various biochemical parameters. Liver and kidney was dissected out for in-vivo antioxidant studies. Pre-treatment of methanolic extract of Saraca indica bark (MESI) in doxorubicin treated group exhibited significant decrease in liver biomarker as well as kidney biomarker. MESI significantly increased the levels of superoxide dismutase (SOD), catalase CAT and reduced glutathione (GSH) while extent of lipid peroxidation (LPO) was significantly decreased in animals treated with DXR. Current study highlights that chronic administrations of extract of Saraca indica bark ameliorated DXR induced hepatotoxicity and nephrotoxicity possibly through its antioxidant action.
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